Microbial biodegradation of biuret: defining biuret hydrolases within the isochorismatase superfamily

Urea and Biuret for Lambs

C ORN silage is a major source of roughage for ruminants in the cornbelt, because it is one of the most productive crops of total digestible nutrients and animal gains per acre. However, to make up a complete growingfinishing ration with corn silage it is necessary to supplement it with sources of energy, nitrogen and minerals. The addition of urea to corn, sorghum or grass at ensiling has give...

Re-evaluation of EDTA-chelated biuret reagent.

We studied the optimal composition of EDTA-chelated biuret reagent for the determination of protein in serum. A reagent containing 18 mmol of EDTA, 15 mmol of Cu2, and 1 mol of NaOH per liter exhibits a very low blankbut about the same sensitivity as tartrate-chelated reagents. Maximum color intensity is attained within 25 mm at room temperature, and adheres to Beer’s law up to 120 g of protein...

The estimation of protein by the biuret and Greenberg methods.

Quantitation of sputum protein by use of the biuret reaction.

A simple, accurate method is described for quantitatively determining the protein content of sputum by use of the biuret reaction. Fitteen mechanically homogenized specimens were examined with the biuret reagent described by Rosenthal and Cundiff, and the results compared with those obtained by the microKjeldahl technique. Over a range of 542 to 1917 mg of protein per 100 ml, the techniques cor...

Determination of protein in urine by the biuret method.

A method for the determination of protein in urine by means of the biuret reaction was described by Hiller (1) and by Hiller, McIntosh, and Van Slyke (2), in which pure biuret prepared by Kahlbaum was used as a standard for visual calorimetry. Since Hiller (1) the biuret method has been used for the determination of urinary proteins by Price (3) and by Lehmann (4), and has been widely used in m...